Determination of aconitase activity
WebDetermination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease Pedro M. Quirós Abstract Mitochondrial aconitase is a reversible enzyme that catalyzes the … WebAconitase 2 ( ACO2) encodes the mitochondrial aconitase (ACO2), an enzyme catalyzing interconversion of citrate into isocitrate in the Krebs cycle. ACO2 mutations have been …
Determination of aconitase activity
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WebThe aconitase activity of a sample may be determined by the following equation: Aconitase Activity = B Sample Dilution Factor (milliunit/mL) T V B = Amount (nmole) of … WebOct 14, 1997 · The aconitase activity was determined in the supernatant using two different methods. Method 1 is a coupled assay in which the formation of NADPH was …
WebDec 13, 2002 · Determination of activities of mitochondrial complexes. For complex activity measurements, samples of synaptosomes (1 mg) were incubated in 1 mL of standard medium for 20 min at 37°C with different concentrations of rotenone, antimycin, or cyanide, respectively. ... Aconitase activity was expressed as a function of the per cent … WebSomething that is curious about aconitase is the fact that it uses iron as the inorganic substituent. Mutanogenic studies on aconitase have found substituting the iron ions with …
WebJul 1, 2007 · We examined the hydroxyketone-mediated production of reactive oxygen species by determination of the aconitase activity. Aconitase is the most sensitive enzyme to reactive oxygen species, and thus, we can estimate the generation of superoxide radical by the inactivation of the enzyme (Gardner and Fridovich, 1992, Murakami and … WebAlper Sarikaya, Aconitase 4 trans mechanism for the reversible dehydration of citric acid as well. From this determination, Gawron et al. surmised that the alpha-hydrogen of these substrates does not participate in the role of aconitase mechanism as the trans mechanism is nowhere near the proximity of the changing subunit. Additionally, the authors found …
WebJun 1, 2015 · The effect of pH on aconitase activity was studied in 100 mM tris–HCl buffer containing 40 mM citrate or 4 mM d, l-isocitrate. Determination of K i was performed by plotting the graphs of dependence of 1/v from the concentration of inhibitor [i] at two fixed concentrations of substrate (citrate) (Dixon, 1953).
WebJul 9, 2004 · Aconitase, a Krebs-cycle enzyme that converts citrate to isocitrate, belongs to the family of iron-sulfur–containing dehydratases whose activities depend on an intact cubane [4Fe-4S] 2+ cluster (1, 2).The purified enzyme is highly susceptible to oxidant-induced inactivation due to release of the solvent-exposed Fe-α and formation of a [3Fe … chiptuning forsikringWebThus, aconitase activity is used as an indicator of levels of intracellular H 2 O 2 and its oxidative damage to cellular components. For example, substantially decreased … chiptuning fiat ducato 244WebDetermination of Aconitase Activity—For the determination of the aconitase activity, cells from the CGXII main culture (∼25 ml) were harvested with ∼25 g of crushed ice … chiptuning ford kuga 1 5 ecoboostWebBeef heart aconitase has been studied by low temper- ature magnetic circular dichroism (MCD) spectroscopy in the wavelength region 300 to I900 nm. Together with parallel electron paramagnetic resonance and ac- tivity measurements, these data enable correlations between Fe-S cluster-type and enzymic activity in aconitase. graphic arts mutual insurance company incWebSep 11, 2014 · In the aconitase activity assay protocol, aconitase catalyzes an equilibrium between aconitate, cis-aconitate and iso-citrate. These reactions are monitored by … chiptuning frlWebDetermination of aconitase activity Cell pellets of the bioreactor samples were washed once with 10 ml washing buffer (20 mM Tris/HCl, pH 8.0) and then resuspended in 4 ml ice-cold lysis buffer (20 mM Tris/HCl, pH 8.0, 5 mM dithio-threitol). Mycelium was broken down by ultrasonic treatment in an chiptuning fs19WebMitochondrial aconitase is a reversible enzyme that catalyzes the conversion of citrate to isocitrate in the tricarboxylic acid cycle. Mitochondrial aconitase is very sensitive to oxidative... Determination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease Springer for Research & Development chiptuning frankfurt