Fitc-conjugated pac-1

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August undefined, 2024

WebCells were blocked with 1% BSA for 30 min and incubated for 60 min with 1:200 dilution of FITC-conjugated rabbit polyclonal anti-C1q antibody (Abcam, Cambridge, MA) and 0.4 μg/ml polyclonal, C-terminal specific rabbit anti-AQP4 antibody (Santa Cruz Biotechnology). Cells were incubated for 30 min with 5 μg/ml goat anti-rabbit IgG-conjugated ... WebKLH conjugated synthetic peptide derived from human AMPD3: Concentration - 100 μg (in 100 μl): 1 mg/ml: Form/Appearance: Lyophilized or Liquid: Conjugation: FITC: MW: 89kDa: UniProt ID: O08739, Q01432, O09178: Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

FITC Anti-C1q antibody (ab4223) Abcam

WebClone HIT3a. Flow cytometric analysis of CD3 expression on human peripheral blood lymphocytes. Whole blood was stained with either FITC Mouse IgG2a, κ Isotype Control (Cat. No. 555573; dashed line histogram) or FITC Mouse Anti-Human CD3 (Cat. No. 561802/555539; solid line histogram). Erythrocytes were lysed with Lysing Buffer (Cat. … WebBecton Dickinson fluorescein isothiocyanate fitc conjugated pac 1 antibody Fluorescein Isothiocyanate Fitc Conjugated Pac 1 Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more ... notes on books

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WebFITC Protocols Fluorescein Isothiocyanate (FITC) - conjugated Antibodies GFP Protocols › Immunoprecipitation Protocols › Membrane Protein Protocols › Protein Expression Protocols › Protein Isolation Protocols › Protein Purification Protocols › SDS PAGE Protocols › WebThe PAC-1 Antibody FITC has been validated for the following applications: Flow Cytometry. Flow cytometry of PAC-1 in PHA-activated human peripheral blood. Sample was incubated with PAC-1 monoclonal antibody (Product # MA5-28564) conjugated to FITC. WebCatalog number: A13199. Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry. Contains 1 vial of annexin V, FITC conjugate. how to set up a charcuterie board

CD41/CD61 Antibody (PAC-1) [FITC] - Novus Biologicals

IHC-plus™ ADCYAP1R1 / PAC1 Receptor Polyclonal Antibody …

WebAug 2, 2010 · The molar ratio of FITC and polymer taken was 1:20. The labeled polymer solution was dialysed against PBS (pH 7.4) for 24 h and then distilled water for 24 h (MWCO 500 for PEI 600, MWCO 1000 for PEI 1800, MWCO 3500 for PEI 25k) until free FITC could not be detected by TLC (chloroform/methanol = 1/1, v/v). ... After conjugation with TA, … WebFeb 16, 2024 · The blood platelets were distinguished based on the expression of CD61. For each sample, 10,000 CD61-positive objects (platelets) were acquired. For the assessment of P-selectin expression, samples were labeled with fluorescently conjugated monoclonal antibody PAC-1-FITC. Results are shown as the percentage of platelets binding PAC … how to set up a charity organisation ukWebDec 1, 2024 · The mono-conjugated FITC-insulin (22%) with unlabeled insulin prepared from 0.25:1 molar ratio (Fig. 3 A), tri-conjugated FITC-insulin (84%) with little amount of di-conjugate prepared from 4:1 molar ratio (Fig. 2 D) and the unlabeled insulin were chosen for the transport study. The percentage amount of mono-conjugate and tri-conjugate … notes on books.html

"WebApr 7, 2024 · And then, they were thoroughly washed, stained orderly with the vinculin antibody (1:500) overnight at 4 °C, the FITC-conjugated goat-anti-rabbit secondary antibody (1:5000) for 1 h, rhodamine-conjugated phalloidin (1:250) for 1 h, and DAPI (1:5000) for 5 min at room temperature. " - Fitc-conjugated pac-1

Fitc-conjugated pac-1

PAC-1 Mouse anti-Human, FITC, Clone: PAC-1, BD - Fisher …

WebThe binding of fibrinogen to the gpIIb/IIIa receptor is required for platelet aggregation. PAC-1 binds only to activated platelets and appears to be … WebColorectal cancer (CRC) is the third most common form of cancer worldwide, with men having a slightly higher incidence rate compared to women. Risk for developing colorectal cancer usually increases with age and can be attributed to inherited genetic mutations along with other comorbidities such as diabetes, IBD, obesity, poor diet, and an ...

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Web2. Slowly add FITC to protein with stirring. Cover with foil and stir 2 hours at room temperature. 3. Separate conjugate from free FITC on G-25 column. Collect fractions. 4. Pool fractions containing conjugate. 5. Determine F/P ratio of conjugate spectrophotometrically. 6. Stabilize with 1% bovine serum albumin and 0.1% sodium … WebFluorescein Isothiocyanate (FITC)-conjugated phal- loidin was obtained from Invitrogen (Invitrogen, Carlsbad, CA, USA). Alexa 488-conjugated anti-rabbit anti-body and 4′,6-diamidino-2-phe- nylindole (DAPI) were obtained from Invitrogen. HPLC solvents were purchased from Fisher Scientific Ltd (Korea). General experimental procedures

WebAnti Pac1 Fitc, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results ... 1) Product Images from "Red blood cell-derived phosphatidylserine positive extracellular vesicles are associated with past ... WebFITC conjugates of antibodies, lectins, hormones, and growth factors have been used in a variety of immunohistochemical and flow cytometry applications. The protocols have been optimized for antibodies, but may be adapted to other proteins by the end user.

WebSep 18, 2024 · FITC-conjugated anti-human CD62P (P-selectin) and FITC-conjugated anti-human PAC-1 were purchased from BioLegend (California, USA). Fluo-3 AM calcium indicators and lactate dehydrogenase (LDH) … Websupplied, and the antibody subclass for each FITC-conjugated antibody. The amount of antibody provided is sufficient for 25 immunostaining reactions using a 1 mL working solution per reaction. Antibody Concentration Amount Subclass Anti-His(C-term)-FITC 1 mg/mL 50 μl (25 reactions)† IgG 2b Anti-myc-FITC 1 mg/mL 50 μl (25 reactions)† IgG 1

WebSpecifications. Name. Anti-PAC-1 Antibody [PAC-1] (FITC) Description. Mouse monoclonal [PAC-1] antibody to PAC-1 (FITC). Specificity. This antibody recognizes an extracellular activation-induced conformational epitope PAC-1 on CD41/CD61 complex (gpIIb/IIIa), also known as integrin alpha IIb beta 3, a receptor which mediates platelet aggregation ...

WebPAC-1 Monoclonal Antibody (PAC-1), FITC, Invitrogen™-Mouse Monoclonal Antibody Shop PAC-1 Monoclonal Antibody ... PAC-1 Monoclonal antibody specifically detects PAC-1 in Human samples. It is validated for Flow Cytometry ... PAC-1: Classification: Monoclonal: Conjugate: FITC: Gene: EIF3EP1: Immunogen: Human platelets: Quantity: 100 Tests ... how to set up a charity on facebookWebphore-conjugated monoclonal antibodies (mAbs): phycoerythrin (PE)-conjugated GPIb (CD42b, Clone HIP1), allophycocyanin (APC)-conjugated P-selectin (CD62P, Clone Psel.KO2.3) (both eBioscience, San Diego, CA, USA), and ﬂuorescein isothiocyanate (FITC)-conjugated PAC-1 (Becton Dickinson Bioscience, San Jose, CA, USA). how to set up a charity raffleWebThe PAC-1 antibody reacts with activation-induced conformational epitope of the integrin a IIb ß 3. PAC-1 binding to fibrinogen is pH and Ca 2+ dependent, and will not bind to platelets that have been fixed. We recommend staining in buffers free of Ethylenediaminetetraacetic acid (EDTA) at a pH of 6.5 or higher. how to set up a charity trustWeb(340507) BD™ FITC Mouse Anti-Human PAC-1 - BD Biosciences - CiteAb. 156 citations – A FITC-conjugated mouse monoclonal antibody, raised against Proteasome assembly chaperone 1 (Human), supplied by BD Biosciences. ... Conjugate FITC Target Proteasome assembly chaperone 1 (Human) UniProt O95456 - PSMG1_HUMAN. Gene PSMG1 … notes on bloodWebProduct Specific Information. The mouse monoclonal antibody PAC-1 recognizes an activation-induced conformational epitope PAC-1 on … how to set up a charity pageWebFITC Conjugated Anti-PAC-1 Monoclonal Antibody (Clone:PAC-1) Share: Figure 1: Surface staining of PAC-1 on PHA-activated (1 hour) human peripheral blood with anti-PAC-1 (PAC-1) FITC. notes on british agarics iiiWebThe antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed. Store undiluted at 4°C and protected from prolonged exposure to light. ... a small organic fluorochrome with an excitation maximum (Ex Max) at 494-nm and an emission maximum (Em Max) at 518-nm. FITC is designed to be excited by the Blue … how to set up a charity golf tournament