Fl83b cells

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August undefined, 2024

WebJul 1, 2014 · FL83B cell morphology before and after DEHP exposure at different concentrations are shown in Fig. 1. With the increase of DEHP concentration, cells tend to shrink. Afterward, the cell lost its ability to adhere on the plate of the dish; thus, it was found floating in the media. Beside shrinkage, cells also showed decrease in cell density. WebAug 1, 2007 · The present study was designed to test the hypothesis that GRK2 negatively regulates IR signaling. FL83B cells, derived from mouse liver, were treated with insulin and membrane translocation of ...

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Web2.3. FL83B cell culture First, the culture method of FL83B cells was slightly modiﬁed from the American Type Culture Collection (ATCC) [18]. The short- and long-term impacts of CLHs on the survivability of FL83B cells in fetal bovine serum (FBS) containing culture sys-tem were determined (Fig. 1A), which could monitor WebFL83B is a hepatocyte cell line derived in 1969 by Charity Waymouth at The Jackson Laboratory, Bar Harbor, Maine from a normal liver taken from a 15-17 day old fetal … how do you spell the name jeremy

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WebApr 25, 2024 · Briefly, FL83B cells (~ 18 × 10 6) were collected by centrifugation and sonicated in cold diluted assay buffer. The supernatant was removed, and cells were re-suspended in cold diluted assay buffer. BHB standard wells and sample wells were prepared as per the protocol. The reaction was initiated by adding developer solution. WebMay 25, 2011 · Finally, the possible mechanism of DPS promoting insulin sensitivity in TNF-α-induced FL83B cells was investigated in this study. DPS may attenuate c-Jun N … WebNormal mouse hepatocyte cell line FL83B (ATCC CRL-2390) was cultured in Ham’s F-12 K medium containing 10% fetal bovine serum (FBS), 100 mg/mL penicillin and 0.25 mg/mL … how do you spell the name ezekiel

GRK2 Negatively Regulates Glycogen Synthesis in Mouse Liver FL83B Cells

WebJul 13, 2024 · FL83B cells were incubated in 400 µM palmitate diluted in growth medium for 24 h. One percent BSA diluted in growth medium was used as control. Moreover, gene knockout was achieved by the CRISPR-Cas9 system, using non-targeting agRNA (control) and Tcf4 or Mafk sgRNA. WebElk-3 siRNA at 20 nM was transfected into TGF-β1-treated FL83B cells (A) or AML12 cells (B). At 48 hours posttransfection, the cells were harvested and lysed. Western blot … how do you spell the name jeanWebJul 13, 2007 · FL83B cells, derived from mouse liver, were treated with insulin and membrane translocation of GRK2 was determined using immunofluoresecence and … phonenumber kit

"WebDec 1, 2024 · Among the subfractions, EAF5-5 markedly enhanced glucose uptake in TNF- α -treated FL83B cells. The possible bioactive compounds of the EAF5-5 fraction that were identified by the HPLC assay include syringic acid, p -coumaric acid, and cirsimaritin. The bioactive compound with the best effect of increasing glucose uptake was p -coumaric … " - Fl83b cells

Fl83b cells

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WebJan 1, 2024 · FL83B cells were grown in F12K medium supplemented with 10% foetal bovine serum (FBS)(Biological Industries, Haemek, Israel) and 100 mg/L penicillin and streptomycin at 37 °C in a humidified atmosphere of 5% CO2. Cells induced lipid accumulation in hepatocytes using 0.5 mM oleic acid (OA) for 48 h, then 3–100 μM … WebApr 1, 1973 · The FL83B cells are morphologically similar to rat liver parenchymal cells [29], to the granular epithelial cells seen on primary explantation of rat liver [30], and to the secondary liver parenchymal cell line obtained by cloning a suspension of rat liver cells [2]. The question of transformation must be addressed in trying to determine the ...

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WebJan 12, 2024 · FL83B cells were co-transfected with pDR1 and pRL-TK (Promega, Seattle, WA, USA) containing CMV promoter-controlled Renilla luciferase gene using fuGENE HD (Promega, Seattle, WA, USA), and incubated with serum free media for 24 hr. Then the cells were treated with FFA and AM as described above, and, another 24 hr later, the …

WebElk-3 siRNA at 20 nM was transfected into TGF-β1-treated FL83B cells (A) or AML12 cells (B). At 48 hours posttransfection, the cells were harvested and lysed. Western blot … WebIn another experiment, FL83B cells were challenged with oleic acid to induce lipid accumulation and treated with various concentrations of fisetin. Results: NAFLD mice treated with fisetin had decreased body weight and epididymal adipose tissue weight compared to NAFLD mice. Fisetin treatment also reduced liver lipid droplet and …

WebFL83B cells were obtained from the Taiwan Bioresource Collection and Research Center (BCRC number: 60325) and cultured in F12K medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco) at 37°C in a humidified atmosphere with 5% CO 2. To make the stock medium containing 4 mM OLA, sodium oleate was conjugated … WebJul 1, 2024 · 2.2. Cell line and treatment. FL83B cells purchased from the Bioresource Collection and Research Center (BCRC, Taiwan). Cells were cultured in F12 medium (Invitrogen-Gibco™, Paisley, Scotland) added with 10% FBS, 2 mM glutamine, 1% penicillin and streptomycin at 37 °C in a humidified atmosphere containing 5% CO 2.Cells …

WebHowever, whether reversibility exists in mesenchymal stromal cell (MSC)-derived hepatocytes (dHeps) remains elusive. In this study, we find that hepatogenic differentiation (HD) of MSCs is a reversible process and is modulated by DNA methyltransferases (DNMTs). DNMTs are regulated by transforming growth factor β1 (TGFβ1), which in turn ...

WebMay 1, 2024 · FL83B cells were seeded on 6-well plates and incubated with 0.5 mM oleic acid for 48 h. The cells were treated with or without tomatidine (0–10 μM) for 24 h. The plate was washed and the cells fixed with formalin solution before staining with Oil Red O solution to assay oil droplets as described previously [25]. Using an inverted microscope ... phonenumbers 8.12.37WebMay 8, 2024 · The effects of a triterpene purified from bitter melon was investigated against TNF-α-induced inflammation via AMP-activated protein kinase in FL83B cells. This compound suppressed the TNF-α-induced expression of inflammatory markers, including inducible NO synthase, p65 subunit of NF-κB, TNF-α, and IL-1β (Cheng et al., 2012). how do you spell the name josephineWebMay 19, 2024 · FL83B cells were seeded in six-well plates at 4x10 5 cells per well with high-glucose DMEM containing 10 vol% FBS, penicillin-streptavidin-amphotericin B (Gibco … phonenumberlookup.comWebDec 1, 2024 · Using the Sephadex LH20 gel packing chromatography column, the EAF5 fraction was isolated from RTL and significantly increased glucose uptake in TNF-α-treated FL83B cells. Moreover, the MCI gel packing chromatography column separated EAF5 into five subfractions and had no significant cytotoxic effect in FL83B cells when treated at … phonenumber tmomail.netWebApr 1, 1973 · The FL83B cells were not disrupted to determine whether VLDL was present intracellularly. In summary, our data show that the FL83B mouse liver cell line grows in a … how do you spell the name katelynWebJan 15, 2024 · FL83B cells were induced as fatty liver cell model by 0.5 mM oleic acid for 48 h, and treated with various concentration of fucoxanthin for 24 h. The results demonstrated that fucoxanthin significantly suppressed lipid accumulation and decreased lipid peroxidation in hepatocytes. how do you spell the name juanWebThe short-and long-term impacts of CLHs on the survivability of FL83B cells in fetal bovine serum (FBS) containing culture system were determined (Fig. 1A), which could monitor cellular morphology ... how do you spell the name josiah