Hu-binding site
Web4 Jul 2013 · The dimer to base pair ratio at 50 nM HU is 1:8. This ratio can be considered as the onset of the high concentration regime. It has been reported that HU binds non-specifically to a 9 bp binding site at high concentrations of HU ( 20, 22, 31). In all aforementioned cases, 50 nM HU (1 dimer: 8 bp), a temporary stiffening is observed, … WebBinding Site provides specialist diagnostic products to clinicians and laboratory professionals worldwide. Our people are dedicated to improving patient lives, …
Hu-binding site
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Web22 Mar 2011 · HU acts to compact DNA at physiological salt concentrations in vitro by introducing bends ranging from 53° to more than 140° in the DNA as assayed by X-ray … WebHU, a major component of the bacterial nucleoid, shares properties with histones, high mobility group proteins (HMGs), and other eukaryotic proteins. HU, which participates in many major pathways of the bacterial cell, binds without sequence specificity to duplex DNA but recognizes with high affinity DNA repair intermediates.
WebHU binding site and present evidence that protein-protein in- teractions do not mediate this high affinity binding. Further- more, we report that the mammalian HMG-1 protein … Web19 Jun 2008 · The histone-like HU (heat unstable) protein plays a key role in the organization and regulation of the Escherichia coli genome. The nonspecific nature of HU binding to DNA complicates analysis of the mechanism by which the protein contributes to the looping of DNA.
WebThe binding sites in c-Fos, interleukin-3, c-Myc, Tau, IVA 2 , and GAP-43 mRNAs are shown. ... Neuronal ELAV/Hu (nELAV) are RNA-binding proteins that mainly regulate … Web1 Mar 2008 · HU binding to ssDNA, dsDNA and nick in low salt conditions. HU protein, at the concentration indicated in nM on the bottom, was mixed with end-labeled DNA, 40-bp long dsDNA, the same DNA with a ...
Web1 Mar 2008 · HU binds single-stranded DNA (ssDNA) in a binding mode that is reminiscent but different from earlier reported specific HU interactions with double-helical DNA …
Web29 Apr 2014 · Site-directed mutagenesis of the putative C/EBPα binding sites decreased FTO promoter activity. Overexpression and RNAi studies also indicated that C/EBP α was required for the expression of FTO. Chromatin immunoprecipitation (ChIP) experiment was carried out and the result shows direct binding of C/EBP α to the putative binding … ugly house to lovely house sign upWeb1 Nov 1996 · The structure of the NSAID binding site is also well conserved, although there are differences in its overall size and shape which may be exploited for the further … ugly house vape pen instructionsWeb15 Jun 1997 · Site-specific binding of HU is promoted by GalR binding and supercoiled DNA Affinity cleavage mapping by chemically converting HU into a nuclease gives information about the position of the reactive groups in HU in the DNA–protein complex ( Lavoie and Chaconas, 1993; Lavoie et al ., 1996 ). thomas hopfner steuerberaterWeb3 Jun 1994 · The bacteriophage Mu in vitro transposition reaction proceeds through several higher order nucleoprotein intermediates (transpososomes). One of the requirements for complex assembly is the Escherichia coli sequence-independent DNA-binding protein, HU. This protein has an affinity for Mu transpososomes, which is at least 100 times greater … thomas hoppmann gothendorfWeb3 Oct 2008 · By contrast, at high concentrations, where HU covers DNA completely, the protein assembles into a rigid superhelical filament on DNA with a persistence length several times that of naked DNA. 11 HU molecules ‘localized’ at designed binding sites in labeled oligonucleotide duplexes appear to bend DNA in solution to the same extent as that in … thomas hopper \u0026 partnersWeb1 Jul 2003 · The binding site size estimated by gel mobility shift experiments for HU is variable. The mesophilic HUs from E.coli and Anabaena are predicted by gel mobility shift analyses to bind from 6 to 10 bp per dimer ( Broyles and Pettijohn, 1986; Bonnefoy and Rouviere-Yaniv, 1991; Cann et al., 1995 ). thomas hopperstad ctHU is able to bind all forms of nucleic acids, including DNA–RNA hybrids (Balandina et al., 2002). Although HU has no specific binding motif, it is able to bind mRNA of RpoS (Balandina et al., 2001). Another study of the B. subtilis HU protein showed that HU binds an Alu domain of cytoplasmic RNA (Nakamura et al., … See more In 1975, a small, low-molecular-weight DNA-binding protein was isolated from E. coli strain U13 (Rouvière-Yaniv and Gros, 1975). At first, it was referred to as Factor U. Due to its … See more HU protein is among the most abundant and conserved nucleoid-associated proteins in eubacteria (Azam and Ishihama, 1999). The greatest abundance of HU protein has … See more Interaction of HU protein at protein level has not yet been fully explored. As mentioned above, an HU protein is able to react with another HU protein to form a dimer together … See more HU seems to be an important protein for DNA compaction, replication, transcription, recombination, and shape modulation in many bacteria (Broyles and Pettijohn, 1986; Roy et al., 2005; Oberto et al., 2009). In the coccoid … See more thomas hopper portal