Phospho-h2ax ser139
WebPhospho-Histone H2A.X (Ser139) (20E3) Rabbit mAb detects endogenous levels of H2A.X only when phosphorylated at Ser139. Species Reactivity: Human, Mouse, Rat, Monkey … WebBecause phosphorylation of H2AX at Ser 139 (γ-H2AX) is abundant, fast, and correlates well with each DSB, it is the most sensitive marker that can be used to examine the DNA …
Phospho-h2ax ser139
Did you know?
WebFeb 17, 2024 · a. Preparation of rabbit anti-phospho-H2AX (Ser139) antibody: Briefly spin the vial of rabbit anti-phospho-H2AX (Ser139) (Item C-1). Add 100 µl of 1X Assay Diluent … WebIntracellular staining protocol for Anti-H2A.X-Phosphorylated (Ser139) Antibody for Flow Cytometry. 1. Prepare 70% absolute ethanol. Chill solution by storing at -20°C. 2. Prepare cells of interest. 3. Wash 1X with PBS, centrifuge at 350g for 5 min. 4.
WebImmunocytochemistry Analysis: A 1:100 dilution of this antibody detected phospho-Histone H2A.X (Ser139) in HeLa cells +/- Staurosporin treatment. Target description 17 kDa observed Physical form Protein G Purified Purified mouse monoclonal IgG1 in buffer containing PBS with 15 mg/mL BSA and 0.1% azide Storage and Stability WebJan 13, 2011 · Herein, we report that EGF strongly induces histone H2AX phosphorylation at Ser139 and at a newly identified phosphorylation site, Ser16, in vitro and ex vivo, and both phosphorylations are mediated by RSK2.Phosphorylation of histone H2AX by RSK2 prevented histone H2AX degradation, and histone H2AX could block cell transformation …
WebAnti-gamma H2A.X (phospho S139) antibody [EP854 (2)Y] See all gamma H2A.X primary antibodies Description Rabbit monoclonal [EP854 (2)Y] to gamma H2A.X (phospho S139) Host species Rabbit Tested applications Suitable for: IP, IHC-P, WB, ICC/IF, Dot blot more details Species reactivity Reacts with: Mouse, Rat, Human Predicted to work with: Sheep
WebThis Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-gamma H2A.X. An anti-pan gamma H2A.X antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and gamma H2A.X present in a sample is bound to the wells by the immobilized antibody.
WebPhosphorylated H2A.X promotes DNA repair and maintains genomic stability. The 2F3 monoclonal antibody reacts with phosphorylated human H2A.X (Ser139) and has been shown to be useful for Western blotting, immunofluorescence and flow cytometry. Product Details Technical data sheet Product Details Verified Reactivity Human, Mouse song chandelier siaWebNov 15, 2024 · RayBio® Phospho-H2AX (S139) and Total H2AX ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important … song chandlerWebAnti-phospho-Histone H2A.X (Ser139), clone JBW301 is a well published Mouse Monoclonal Antibody validated in ChIP, ICC, IF, WB. This purified mAb is highly specific for phospho … small electrical appliances albox spainWebBethyl Laboratories Anti-Phospho-gamma-H2AX (Ser139) Polyclonal, Catalog # A300-081A. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and Immunohistochemistry … song chandelier lyricsWebNov 15, 2024 · RayBio® Phospho-H2AX (S139) and Total H2AX ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated H2AX protein in your experimental model system, you can verify pathway activation in your cell lysates. small electrical appliance repairs perthWebAnti-phospho-Histone H2A.X (Ser139), clone 6L16 ZooMAb, is a recombinant Rabbit Monoclonal Antibody that specifically targets phospho Histone H2AX (ser139) and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Peptide Inhibition Assay, and Western Blotting. song change is gonna comeWebNov 15, 2024 · Cells were then incubated with phospho-histone H2A.X (Ser139) antibody (from Cell Signaling Technologies 9542, Boston, MA, USA) for 1 h, followed by DAPI … song change change change